Irreversible inhibition of the interaction between elongation factor Tu and phenylalanyl transfer ribonucleic acid by L-1-tosylamido-2-phenylethyl chloromethy ketone.

نویسندگان

  • N Richman
  • J W Bodley
چکیده

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GTPase center of elongation factor Tu is activated by occupation of the second tRNA binding site.

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Competition between the elongation factors 1 and 2, and phenylalanyl transfer ribonucleic acid for the ribosomal binding sites in a polypeptide-synthesizing system from brain.

80 S ribosomes and elongation factors 1 and 2 (EF-1 and EF-2), isolated from freeze-dried porcine brain of the hypothalamic region, were used to study the interrelation between the ribosomal binding of the two factors and of phenylalanyltRNA and N-acetyl-phenylalanyl-tRNA. Complexing of ribosomes with EF-2 + /3, y-methylene-guanosine S’-triphosphate or EF-2 + GTP + fusidic acid prevented or inh...

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Competition between the Elongation Factors 1 and 2, and Phenylalanyl Transfer Ribonucleic Acid for the Ribosomal Binding Sites in a Polypeptide-synthesizing System from Brain*

80 S ribosomes and elongation factors 1 and 2 (EF-1 and EF-2), isolated from freeze-dried porcine brain of the hypothalamic region, were used to study the interrelation between the ribosomal binding of the two factors and of phenylalanyltRNA and N-acetyl-phenylalanyl-tRNA. Complexing of ribosomes with EF-2 + /3, y-methylene-guanosine S’-triphosphate or EF-2 + GTP + fusidic acid prevented or inh...

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 248 1  شماره 

صفحات  -

تاریخ انتشار 1973