Irreversible inhibition of the interaction between elongation factor Tu and phenylalanyl transfer ribonucleic acid by L-1-tosylamido-2-phenylethyl chloromethy ketone.
نویسندگان
چکیده
منابع مشابه
Inhibition of initiation of protein synthesis in mammalian tissue culture cells by L-1-tosylamido-2-phenylethyl chloromethyl ketone.
Incorporation of amino acids into proteins in HeLa cells, virus-transformed 3T3 mouse fibroblasts, and mouse plasmacytoma cells is inhibited after the addition of L-1-tosylamido-2-phenylethyl chloromethyl ketone, an alkylating agent and chymotrypsin-specific protease inhibitor. Addition of this drug to tissue culture cells at concentrations of 20 to 30 mug per ml results in an irreversible inhi...
متن کاملInhibition of Initiation of Protein Synthesis in Mammalian Tissue Culture Cells by L-1-Tosylamido-2-phenylethyl Chloromethyl Ketone
Incorporation of amino acids into proteins in HeLa cells, virus-transformed 3T3 mouse fibroblasts, and mouse plasmacytoma cells is inhibited after the addition of L-l-tosylamido-Z-phenylethyl chloromethyl ketone, an alkylating agent and chymotrypsin-specific protease inhibitor. Addition of this drug to tissue culture cells at concentrations of 20 to 30 c(g per ml results in an irreversible inhi...
متن کاملGTPase center of elongation factor Tu is activated by occupation of the second tRNA binding site.
Interaction of the elongation factor EF-Tu with the antibiotic kirromycin results in activation of the GTPase center of the factor and in induction of an additional tRNA binding site (tRNA binding site II to distinguish it from the classical tRNA binding site I). Activation of the GTPase center under these conditions is stimulated by addition of tRNA. Two-fold evidence is presented that this st...
متن کاملCompetition between the elongation factors 1 and 2, and phenylalanyl transfer ribonucleic acid for the ribosomal binding sites in a polypeptide-synthesizing system from brain.
80 S ribosomes and elongation factors 1 and 2 (EF-1 and EF-2), isolated from freeze-dried porcine brain of the hypothalamic region, were used to study the interrelation between the ribosomal binding of the two factors and of phenylalanyltRNA and N-acetyl-phenylalanyl-tRNA. Complexing of ribosomes with EF-2 + /3, y-methylene-guanosine S’-triphosphate or EF-2 + GTP + fusidic acid prevented or inh...
متن کاملCompetition between the Elongation Factors 1 and 2, and Phenylalanyl Transfer Ribonucleic Acid for the Ribosomal Binding Sites in a Polypeptide-synthesizing System from Brain*
80 S ribosomes and elongation factors 1 and 2 (EF-1 and EF-2), isolated from freeze-dried porcine brain of the hypothalamic region, were used to study the interrelation between the ribosomal binding of the two factors and of phenylalanyltRNA and N-acetyl-phenylalanyl-tRNA. Complexing of ribosomes with EF-2 + /3, y-methylene-guanosine S’-triphosphate or EF-2 + GTP + fusidic acid prevented or inh...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 248 1 شماره
صفحات -
تاریخ انتشار 1973